Cst myc antibody
WebACTL6B/BAF53B is a member of the neural-specific SWI/SNF complex that facilitates binding to target genes and is involved in memory and synaptic plasticity (13-15). ACTL6/BAF53 has been shown to interact with c-Myc, where it functions as a cofactor and is important in the transformation process (16). Further studies have shown … Webc-Myc Antibody. Applications: WB, IHC, ICC/IF, IP, ELISA. Reactivity: Human, Mouse, Rat, Tag, Feline. Publications: 749. Images: 417. ... The c-Myc protein is a 62 kDa …
Cst myc antibody
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WebGet better batch-to-batch reproducibility with a recombinant antibody. Anti-c-Myc (phospho S62) antibody [EPR17924] (ab185656) Research with confidence – consistent and reproducible results with every batch. Long-term and scalable supply – powered by recombinant technology for fast production. Success from the first experiment – … Web6x-His Tag Antibody (MA1-21315) in ICC/IF. Immunofluorescent analysis of 6x-His Tag was performed using 70% confluent HEK-293 cells transfected with V5-H3-His construct. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature ...
WebAnti-Myc Tag, clone 4A6, is a mouse monoclonal antibody that is validated for use in ChIP, IC, IF, IP and WB for the detection of Myc Tag and does not show the very high context sensitivity of 9E10. This antibody has been cited in over 480 publications. Research Category. Epitope Tags & General Use. Research Sub Category. WebFeb 2, 2007 · DOI: 10.1002/prot.22080. Primary Citation of Related Structures: 2OR9, 2ORB. PubMed Abstract: The X-ray structure of the Fab fragment from the anti-c-myc …
WebAt CST, we adhere to the Hallmarks of Antibody Validation™, six complementary strategies for determining the specificity, sensitivity, and functionality of an antibody in any given assay. By carefully tailoring these strategies to each antibody product, we guarantee that CST antibodies will work as expected, to help you achieve results you ... WebThe mouse Anti-c-MYC monoclonal antibody recognizes an epitope located within a sequence (EQKLISEEDL) of the product of the human oncogene c-myc. c-Myc (v-myc avian myelocytomatosis viral oncogene homolog) is a transcription factor, which is coded by MYC gene. It was first described as a homolog of an avian retroviral oncogene.
Webc-Myc (also known as Myc), together with l-Myc and n-Myc, belongs to the Myc family of transcription factors. c-Myc has a basic helix-loop-helix leucine zipper domain and through heterodimerization can bind and regulate the transcriptional activity of genes, either by repression or activation. It is a key player in the regulation of cell growth ...
WebICC staining of purified anti-c-Myc antibody (clone 9E10) on HeLa cells transfected with multi-tag plasmid containing c-Myc epitope sequence. The cells were fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100, and blocked with 5% fetal bovine serum in PBS for 1 hour. The cells were then incubated with 1 µg/mL of the ... onyx residenceWebThe immunogen of 16286-1-AP is MYC tag Fusion Protein expressed in E. coli. PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC … onyx rip free downloadWebMonoclonal Antibody for studying Myc. Cited in 820 publications. Validated for WB, WB, IF. Available in 2 sizes. Highly specific and rigorously … onyx reserve robustoWebApr 11, 2024 · Anti-CK2α Antibody: Cell Signaling: 2656S: Anti-Puromycin (12D10) antibody: Millipore: MABE343: Secondary antibodies: Rabbit IgG HRP linked: GE Healthcare: NA934: Mouse IgG HRP linked: ... before immunoprecipitation with 4 μg of anti-Myc-tag antibody (Cell Signaling) per sample, preconjugated to protein G Dynabeads … iowa basketball fan forumsWebHuman c-Myc is a helix-loop-helix transcription factor which efficiently binds DNA after heterodimerization with the bHLH protein Max. It is often overexpressed and mutated in hematopoietic tumors. Mutations frequently result in truncation around amino acid (aa) 252, before the C-terminal DNA binding, HLH and leucine zipper domains. onyx rewards member rateWebCells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 3% BSA for 1 hour at 22°C. Samples were incubated with primary antibody (1/1000 in diluent) for 1 hour at 22°C. A FITC-conjugated goat anti-mouse polyclonal IgG (1/1000) was used as the secondary antibody. Nuclei were stained with DAPI (blue). See ... onyx rewardsWebThe FLAG tag (peptide sequence DYKDDDDK) is a short, hydrophilic protein tag commonly used in conjunction with antibodies in protein pull-downs to study protein–protein interactions. The FLAG tag may be inserted at the N terminus, the N terminus preceded by a methionine residue, the C terminus, or internal positions of the target protein. onyx rewards halo infinite