site stats

Gm-130 antibody-directed staining

WebAnti-GM130 (green) antibodies identified GM130-expressing and GM130-depleted cells. Nuclei were counterstained in blue. When compared with non-transfected cells visible on the same field, merged apotome views showed higher giantin staining in cells positive for FLAG tag (arrowheads), and lower giantin staining in cells negative for GM130 ... WebThe 35/GM130 monoclonal antibody recognizes GM130, regardless of phosphorylation status. Skip to ... BD Horizon™ Brilliant Stain Buffer. RY586 Reagent Promo. Lab Week 2024. View All Promotions. …

GOLGA2/GM130 antibody (CL488-11308) Proteintech

WebApr 6, 2024 · Immunogold staining was employed to detect the PI4P on sEVs. The results showed that the PI4P antibody labels were observed on the sEVs. The GFP labels of the GFP-tagged PI4P sensor were also observed on sEVs, while the secondary antibody negative control was not labelled (Figure 1g). The antibody cannot penetrate the cell … WebIn biochemistry, immunostaining is any use of an antibody -based method to detect a specific protein in a sample. The term "immunostaining" was originally used to refer to the immunohistochemical staining of tissue … the karaoke channel app for sony smart tv https://constantlyrunning.com

GM130 Antibody (H-7) SCBT - Santa Cruz Biotechnology

WebIntracellular flow cytometry applications for GM-CSF Antibody, anti-human APC PE Conjugate: APC Recommended dilution: 1:10 Remark: The antibody is suited for staining of formaldehyde-fixed cells. Tested: QC tested Products used: 130-099-625, 130-096-078 Protocols: Intracellular flow cytometry staining protocol (Inside Stain Kit 1:10, protocol A) WebImmunofluorescent staining of human cell lines. HeLa cells (ATCC CCL-2) were cultured, fixed, permeabilized with Triton™ X-100, stained with Alexa Fluor® 488 Mouse anti-GM130 (pseudo colored green), and counter stained with Hoechst 33342 (pseudo colored blue) according to the Recommended Assay Procedure. WebIntroduction. Cold-antibody autoimmune hemolytic anemias (cAIHAs) are mediated by autoantibodies characterized by a temperature optimum of the antigen-antibody (AgAb) … the karankawas facts

Recombinant Alexa Fluor® 488 Anti-GM130 antibody [EP892Y ... - Abcam

Category:Antibodies Free Full-Text An Antibody Targeting Fibroblast ...

Tags:Gm-130 antibody-directed staining

Gm-130 antibody-directed staining

GM130 Antibody RQ4570 NSJ Bioreagents

WebRabbit anti-β-catenin (C-2206) antibody, that the observed upregulation of VE-cadherin and ETS1 mouse anti-N-cadherin (C3865) and mouse anti-β-actin expression via NF-κB is part of the transcriptional pro- (A-5441) antibodies were obtained from Sigma-Aldrich. gram that endothelial cells implement to tune vascular Mouse anti-VE-cadherin (BV6 ...

Gm-130 antibody-directed staining

Did you know?

WebGolgi Matrix Protein (GM130) is peripheral cytoplamic protein that is bound to the Golgi membranes. It has been implicated as a maintainer of cis-Golgi structure (1). During mitosis, it regulates the disassembly and reassembly of the Golgi complex. WebDec 5, 2024 · Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GM130 Antibody (H-7) has 22 citations in a variety of scientific publications. …

WebGOLGA2, also known as GM130, is a 130 kDa cis-Golgi matrix protein which is one component of the detergent and salt resistant Golgi matrix. It is a peripheral membrane protein highly bound to Golgi membrane and … WebRabbit Polyclonal GM130 antibody. Validated in WB, ICC/IF, IP. Tested in Human. Cited in 3 reference(s). United States (US) Country / Location Selection Product Research ... Blue: Hoechst 33342 staining. GTX130351 IP Image Immunoprecipitation of GM130 protein from HeLa whole cell extracts using 5 μg of GM130 antibody (GTX130351). ...

WebNormal Function. Collapse Section. The BICD2 gene provides instructions for making one of a family of proteins called golgins. Golgins help maintain the structure of a cell … WebII. Primary Antibody Incubation. Incubate membrane and primary antibody (at the appropriate dilution and diluent as recommended in the product webpage) in 10 ml …

WebDec 5, 2024 · GM130, a cis-Golgi matrix protein, interacts specifically with p115 and provides a membrane docking site. Both GM130 and p115 are involved in vesicle tethering to Golgi membranes. The amino-terminus of GM130 binds to p115, whereas the carboxy-terminus binds to Golgi membranes. Both Giantin and GM130 compete for binding to p115.

WebWhen antibodies bind to or block these targets they disrupt functioning, resulting in neurologic and psychiatric symptoms. 6. The autoantibodies may also trigger inflammation (encephalitis) in the basal ganglia. This … the karaoke chilla artWebApr 17, 2016 · Then, on the next day, the neurons were loaded with Fluo-4 calcium indicator and imaged for 5 mins. KCl treatment was also used as a positive control, but … the karakoram highway chinaWebAntibodies that detect GM130 can be used in several scientific applications, including Western Blot, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation and Flow Cytometry. These antibodies target GM130 in Human, Rat, Mouse and Canine … the karanga dance is characteristic ofWebGOLGA2, also known as GM130, is a 130 kDa cis-Golgi matrix protein which is one component of the detergent and salt resistant Golgi matrix. It is a peripheral membrane … the karaleeWebMay 15, 2024 · Antibodies used at too high of a concentration can result in off-target staining.32–35 While determination of signal to noise ‘by eye’ is often used, to be … the karan menonWebKey features and details. Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply. Rabbit … the karat shop westbury nyWebBuy gm130 antibodies from Santa Cruz Biotechnology, Inc. Monoclonal antibodies are available to most protein immunogens. Choose a Store ... CONTROL SERA FOR IHC … the karaites