Myotube diameter length number
WebAug 15, 2024 · Linear regression is shown, using the least-squares method (N = 32, where N represents the number of myotubes, r = 0.64, P < 0.05). The length of wrinkles is … WebJan 24, 2024 · The number, diameter, length, maturation index, and coverage of myotubes were quantified by morphological analysis (Figs. 6b–6g). The results indicated clear differences in all groups with the Patch/SMF1 group showing the highest maturation rate, while cells in the control group had barely differentiated.
Myotube diameter length number
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WebJan 22, 2024 · ( E and F) Quantified (E) projected myotube length ( n > 150 myotubes from two donors per group) and (F) SAA cross-striation frequency ( n ≥ 30 images from three donors per group) from longitudinal sections of myobundles. * P < 0.05 versus IFN-γ −, #P < 0.05 versus IFN-γ +. NS, nonsignificant. Data are presented as means ± SEM. Open in viewer Webtube size is the calculation of average myotube diameter derived from multiple measurements made along their length (Rommel et al. 2001; Jaquemin et al. 2004; Ohanna ... traced, and the number of pixels corresponding to its length was given by the software. When measurements were made subsequently, the software automatically converted …
WebJun 4, 2013 · (C) Dose-dependent reduction in numbers and lengths of C2C12 myotubes at 48 h and 72 h. Arrows indicate myotubes. (D) Dose-dependent reduction in C2C12 myotube length, diameter, and area at 72h. (E) Dose-dependent effect of IFN-β on 72 h human skeletal muscle with marked inhibition of myotube formation at 100 U/ml.
WebJan 17, 2024 · While the myotubes in Serum culture presented the largest diameter (23 ± 5 µm), those of AIM-V and B27 had smaller diameters (13 ± 2 µm). All myotubes showed contraction movement at day 7,... WebAdministration of 3,5-DHBA also increased myotube diameter. Not only lactate but also 3,5-DHBA upregulated the phosphorylation level of mitogen-activated protein kinase kinase 1/2 (MEK1/2), p42/44 extracellular signal-regulated kinase-1/2 (ERK1/2) and p90 ribosomal S6 kinase (p90RSK). MEK inhibitor U0126 depressed the phosphorylation of ERK ...
WebSodium lactate (lactate) or GPR81 agonist, 3,5-dihydroxybenzoic acid (3,5-DHBA), was administered to the differentiation medium. Results: Lactate administration increased the …
Webi this article, we present two techniques designed to evaluate n changes in myotube diameter applicable to both low- and high- throughput screening. the first method is a … gfn incWebApr 17, 2024 · Using Image J, the diameters of at least 100 myotubes in each well were measured at three randomly selected portions taken along the length of the myotube. Then, the average diameter of a myotube was calculated as the mean of three measurements. gfn libraryWebThe average length, diameter, area (Fig. 2E-G) as well as maximum width (data not reported) of myotubes were 1.3-3 fold higher in ICAM-1þ compared to control and empty vector cells at and area... christoph-probst-weg 4 20251 hamburgWebAug 1, 2013 · The fabricated 18 × 18 mm submicron-grooved surface was 200 nm in depth, 400 nm in width and 800 nm in space. Prior to seeding cells, submicron-grooved PS … gfn it trainingWebJun 9, 2024 · Myotube formation and morphology was quantified with the following parameters: myotube length (a), alignment (b), diameter (c) and myotube density (d). … gfn hogwarts legacyWebmyotube growth in the presence of PA was evaluated. Compared to control cells, 150 M PA reduces myoblast proliferation and the expression of MyoD and miR-206 and miR-133a … gfn free premiumWebJan 10, 2024 · The average diameter per myotube was calculated as the mean of 5 measurements taken along the length of the myotube. Morphological analyses were performed using ImageJ software. The fusion index was determined by dividing the total number of nuclei in myotubes by the total number of nuclei counted (Ren et al. 2008). … christoph promberger wikipedia